FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8271863
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG249590.30173372068662163No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG189150.038.03180770
CCGTATC355650.032.60308555
CGTATCA357200.032.3732356
CGCCGTA365950.032.3654653
GCCGTAT375400.031.67192554
TCGCCGT375450.031.52787252
GTCGCCG396100.031.0682851
GTATCAT364500.030.87011557
GGTCGCC406000.030.67277550
TGGTCGC406400.030.10862449
CATTAAA403350.029.76974361
ATCTCGG380450.029.64592442
TCATTAA399250.029.6282260
CTCGGTG378600.029.57855244
GATCTCG379250.029.43558341
TCTCGGT379300.029.41289343
AAGATCG164700.029.0023676
AGATCTC390550.028.9871340
CGCGTAG4000.028.8823262
GTGGTCG424800.028.79537648
Produced by FastQC (version 0.11.5)