FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4786468
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155850.32560543599163305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG148050.039.2746570
CGAGATC38100.034.809115
AAGATCG177000.034.18086
AAAGGGG281200.033.33852470
CGCCGTA373300.033.24022753
CCGTATC367950.033.20914555
CGTATCA368100.033.09982756
TCGCCGT398750.032.91821752
GTCGCCG435650.032.86179751
GCCGTAT378150.032.77687554
GGTCGCC440500.032.69795250
CGTGTCA29300.032.37346656
TGGTCGC424450.032.30157549
GATCTCG399050.032.19075841
ATCTCGG402650.032.09380342
CCGTGTC31900.032.03972655
CTCGGTG403350.031.94334444
GTATCAT368400.031.88525457
AGATCTC403050.031.86260240
TCTCGGT401750.031.79117443