FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5121046
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114070.22274746213957072No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG105520.20605165429093977No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA89440.17465181917912864No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC89380.1745346556152786No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC67990.13276584510273878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG11350.024.3899781
TGTCGGC31500.023.33348730
GTGGGAC31900.022.8232519
CGAGCAG10300.022.7938541
TCGGCGG33450.021.65912232
GTCGGCG33750.021.25919231
CCGTATC65450.020.85575155
CGGCGGC35600.020.54768833
CGTATCA66600.020.39052256
GAATGTC37350.020.0554427
CGCCGTA69750.019.97223953
CGGCCAG3200.019.710731
TGCGGTG8750.019.6231271
AATGTCG37750.019.56476228
CGGGCAG3800.019.3649271
CGCGAAG8000.019.272711
CGGGAAG85000.019.25211
GTATCAT68700.019.15587457
GAAGCGT40500.018.92919369
ATGTCGG38650.018.9280529
Produced by FastQC (version 0.11.5)