FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118778
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85870.2753321974183478No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA51320.1645516288751556No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC49380.15833124383973465No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG40610.13021125581878543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC16150.028.17275630
GTGGGAC17450.026.4767619
GAATGTC19350.024.60091427
CGGCGGC18700.024.51817733
ATGTCGG19300.023.93921529
CGCGAAG4650.023.3661631
CGCCAAG5500.022.3040681
TCGGCGG20500.022.19463332
GTCGGCG20500.022.19463331
ACGCGGT6800.022.13044737
CGAGCAG5100.021.9916861
CGGGAAG38050.021.9230521
AATGTCG21300.021.52707528
CGCCGTA12450.021.08421353
CACCTCG4150.021.08387434
CGGCAAG6650.021.0822541
CCTGCCG3650.020.13492839
GGCGGCA22900.019.86855934
CCCTCAG3700.019.86506552
CTAATCG3900.019.74252313
Produced by FastQC (version 0.11.5)