FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6641944
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206920.31153529749723874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC19950.027.192630
CGCGAAT4900.024.311551
CGCGAAG6750.023.3581561
CGGCAAG12800.022.1719991
GTGGGAC25100.022.0333919
CCCGGCG5800.021.11991716
TCGGCGG26150.020.87924832
CGGGAAG54600.020.8554951
GAATGTC26050.020.42397927
CGGGCAG5850.020.3635221
TGCGGTG12250.020.3072951
CGAGCAG9150.020.2947921
AATGTCG27100.020.14929228
TAGTCCG25600.019.54933211
GTCCGCA25700.019.47384813
AGTCCGC25750.019.43545212
GCCCGGC6350.019.29048715
CAGCCCG6950.019.13584713
AGCCCGG6450.018.99141114
CGGCAGT30300.018.71176536
Produced by FastQC (version 0.11.5)