FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5849443
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG219910.3759503255267211No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA66640.11392537716839021No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC63800.10907021403576374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC18900.026.8524530
CGGCAAG8950.025.8342631
CGAGCAG9000.025.3014851
CGCCAAG8200.025.2065451
CGGCATA4800.024.0850681
GAAGCGT27000.022.42968469
GTGGGAC23050.022.17063919
GAATGTC23700.021.71027227
CGGCGGC23850.021.42605433
AAGCGTA28550.021.33457470
AGTCCGC30850.021.21385212
GTCCGCA30900.021.18025413
TAGTCCG31250.021.05430811
CGGGAAG43300.020.631341
TCGGCGG24500.020.57188632
TGAAGCG30250.020.25115268
GGCGGCA25650.020.19520834
TGCGGTG9850.019.917141
CGCGAAT3700.019.8834951
CGGCAGT26950.019.35008636
Produced by FastQC (version 0.11.5)