FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8797348
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265390.30167045796074No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG106010.12050222407934755No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA89980.10228082372096682No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC88920.10107591515079319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC32600.024.37107330
CGGCGGC35700.022.35285633
CGGCAAG21750.021.5874331
CGCGAAG11050.021.5626491
GTGGGAC38750.020.6859119
TCGGCGG39450.020.40549932
CGAGCAG24250.020.2288781
CGGGAAG94400.020.1921421
GTCGGCG39900.020.1753631
GAATGTC43300.018.7548427
CGCGGAG7700.018.2022361
CGTGACC5000.018.1993
TGCGGTG15100.018.0997751
CTAAACG53750.017.6483521
AATGTCG45550.017.52103428
TTAACCG52500.017.2656258
GCGGCAG46000.017.11813535
CGCGAAA14150.017.0863061
GGCGGCA46950.016.99664334
ATGTCGG47950.016.7900729
Produced by FastQC (version 0.11.5)