FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875291
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69240.24081040840735773No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC29110.10124192646935562No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACACC9200.036.517454
GGAACAC10700.032.38053
CGCGGAT1650.029.7265681
CGGCCTG1451.8189894E-1128.9943831
TGTCGGC8050.028.69577430
TGCGTCG2400.027.7359491
CGGCAAG6600.026.5415781
CCGCCCG808.66666E-526.25376567
CGGCTAG1601.8462742E-924.0864831
CAGGAAC14950.023.6690521
CGGCGGC10350.022.657133
GTGGGAC10200.022.64833619
TCGGCGG10900.021.51385132
CGCGAAT2950.021.3772141
CGCCAAT5450.021.2137811
CGGGAAG19900.020.9505061
GTCGGCG11200.020.62508831
GTCCGCA12050.020.04053313
CGCGCAG1055.432711E-420.0199321
CGCGAAA4200.020.0199321
Produced by FastQC (version 0.11.5)