FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404931
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC12310.3040024102871847Illumina Single End PCR Primer 1 (100% over 50bp)
CGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG8780.21682706436405214Illumina Single End PCR Primer 1 (100% over 30bp)
CGTCGTGTAGGGAAAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT7690.18990889805917552Illumina Single End PCR Primer 1 (100% over 31bp)
CGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGA7420.18324109539649963Illumina Single End PCR Primer 1 (100% over 29bp)
CGTCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG7230.17854893796720922Illumina Single End PCR Primer 1 (100% over 32bp)
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA5960.14718556988721535No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC5700.14076472287871267No Hit
GTGCAAGATGAATAGCCAGTGCAATATATACATGTATACTTAACAGATAT5300.13088649671178545No Hit
GGGAAAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC5100.12594738362832186Illumina Single End PCR Primer 1 (100% over 40bp)
GCGGCAGTATTGAGACCATGAGAGTAGCAAACGTAAGTCTAAAGGTTGTT4630.11434046788218241No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG4630.11434046788218241No Hit
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG4630.11434046788218241Illumina Single End PCR Primer 1 (100% over 32bp)
AGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC4490.11088308872375786Illumina Single End PCR Primer 1 (100% over 40bp)
AGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC4180.1032274634443893Illumina Single End PCR Primer 1 (100% over 38bp)
TCGTGTAGGGAAAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT4140.10223964082769657Illumina Single End PCR Primer 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA3300.064.74761
ACGTATG3450.060.8494833
CGTATGG3800.057.0864264
GCGCGTT252.3887007E-456.00227762
AACGTAT3750.055.981522
CAGACCC200.00695107952.49564716
CCGCGCG451.6825961E-646.66856460
CGCGCGT305.873689E-446.6685661
TGTCGGC1500.039.66827830
CCCCGCA459.189137E-538.8904748
GTTCCCG459.189137E-538.8904756
TCCCGCG459.189137E-538.8904758
CCCGCGC459.189137E-538.8904759
CGGCAAG1007.2759576E-1238.5301251
CGCCGAA556.652641E-638.2116931
TGTCGTG2500.036.4284821
CGCGAAA400.00240832835.0273861
CGCACAG708.628431E-735.0273861
TCGCGTA501.70069E-435.0273861
GCCCGGG400.002414177435.0100769
Produced by FastQC (version 0.11.5)