FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8714845
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG242610.2783870510605754No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC99750.11445986704295946No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA97540.11192396422426332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG143500.029.0521770
CCGTATC201000.028.5911155
CGTATCA202950.028.36813556
CGCCGTA206400.028.33597253
GCCGTAT214650.027.31211354
TCGCCGT219600.027.04726452
GTCGCCG236800.026.29469551
GGTCGCC242300.025.91435450
GTATCAT222200.025.15444657
ATCTCGG232850.025.1438242
TGGTCGC243150.025.06085649
CTCGGTG231250.024.92489844
GATCTCG234300.024.74937841
TCTCGGT232450.024.73543543
AGATCTC242200.024.115540
TGTCGGC39350.024.0147430
GTGGTCG255650.023.94502848
CATTAAA258150.023.33190561
GGTGGTC264300.023.09474647
TCATTAA259100.022.94919460
Produced by FastQC (version 0.11.5)