FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842072
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58230.31611142235482653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA552.2719178E-950.9511681
CGCGCAG1450.048.3157581
AAAGGGG204250.047.8542670
AAGGGGG66200.042.04132570
CGAGATC36150.041.3331225
GCAGATC31800.039.8345644
CTAGATC31100.038.7058535
CCGAGAT6000.038.4920734
TCGAGAT12000.038.2004664
CGCATAG1750.038.0004272
CGTGAGA6450.037.98083
GGGTCGC10050.037.61338449
GGCAGAT12200.037.5783123
AAGATCG176800.037.447046
AAAGATC61850.037.1715
GCTAGAT19150.036.9114574
CGCTAGA6800.036.5405583
CGGCAAG3850.036.3936841
TCGCGTA1450.036.236821
CCGTATC435550.035.7517555
Produced by FastQC (version 0.11.5)