FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316334
Sequences flagged as poor quality0
Sequence length76
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38470.29225105482347186No Hit
CGGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG18610.1413774923385706Illumina Single End PCR Primer 1 (100% over 45bp)
CACGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG17820.13537597600608964Illumina Single End PCR Primer 1 (100% over 44bp)
TTTGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG16700.1268674971549774Illumina Single End PCR Primer 1 (100% over 44bp)
TTCGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG16640.12641168578795353Illumina Single End PCR Primer 1 (100% over 44bp)
CGCGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG16060.1220055092400561Illumina Single End PCR Primer 1 (100% over 44bp)
CGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG15670.11904273535440094Illumina Single End PCR Primer 1 (100% over 45bp)
CGCGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG15060.11440865312299159Illumina Single End PCR Primer 1 (100% over 45bp)
TGCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG14380.10924279096338771Illumina Single End PCR Primer 1 (100% over 44bp)
CGCAAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG14360.10909085384104643Illumina Single End PCR Primer 1 (100% over 44bp)
CGCAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG13950.10597614283304997Illumina Single End PCR Primer 1 (100% over 45bp)
CCGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG13690.1040009602426132Illumina Single End PCR Primer 1 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC150.002224698470.002254
CGGTCCC150.002224698470.002256
TCCGTCG150.002226039569.9915745
CGGCCAG800.065.699451
CGCACGA501.4551915E-1163.071471
CACGCTA1900.062.7026331
CACGCAA2550.061.8347741
CGCGTGA1250.061.6698841
TGCGGTA1650.061.5849381
CGCGAAA2050.061.5331421
CCCGCGA401.0577423E-861.3194851
TCCGCAA1650.059.461321
CGGCCTA650.059.2979621
CGCATAG1800.058.350692
TCGCGTA1700.057.712461
CGCCAAA1700.057.712461
TTCGCGA3100.057.645971
CGCGATA553.6379788E-1157.33771
TTCCCGA1600.056.939521
TTCACGA2600.056.60261
Produced by FastQC (version 0.11.5)