FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8031305
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270410.33669497049358726No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG83230.10363197512732987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC22600.022.76490830
CGGGAAG76750.019.9488241
TTAACCG42300.018.9469258
CGGCGGC27000.018.79581833
GTCGGCG27750.018.79231531
CTAAACG44250.018.5889521
TCGGCGG28850.018.31842432
TAGTCCG22900.018.18640311
AGTCCGC23150.018.14118412
CGGCAAG20200.018.0383241
GTCCGCA23250.017.91341213
GTGGGAC29400.017.85846719
CGCGGAG6350.017.6559261
TAACCGT46400.017.34815659
CGAGGGT48950.016.8042126
ACGAGGG48300.016.74047725
CGCGAAA10450.016.4283521
CGCACAG6250.016.2566951
GCAACTA50450.016.23398817
ACCGTAG48800.016.208161
Produced by FastQC (version 0.11.5)