FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5358305
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206440.3852710885251959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAG9100.023.4809911
TGTCGGC15550.022.05844530
CGGCAAG10200.021.6355691
CGGGAAG37650.019.9102521
TGCGTCG7200.019.4605661
GTCCGCA21750.018.98793213
AGTCCGC22150.018.96069712
TGCGGTG9300.018.8328041
TGTCGTG16400.018.7960591
CGCGAAA7300.018.7141321
TCGGCGG18750.018.48047432
TAGTCCG22950.018.1472611
TCGGGAA12400.018.0794931
CGCGGAT3203.6379788E-1217.5145071
GTGGGAC20250.016.93965519
CGCCCAC2301.1986958E-716.7530081
TCCGCAT24250.016.74176214
TCGCGTA3359.094947E-1216.7302781
CGGCGGC21050.016.46123133
TGCAGGT14100.016.396561
Produced by FastQC (version 0.11.5)