FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8699114
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286570.32942435287087857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC25350.027.88971130
GCGAGCG18000.025.08146711
GGCGAGC18700.024.32736210
CGGCCTA2250.023.3579121
CGGCGGC30650.023.29540833
CGGCAAG15500.023.056521
CGAGCGT20000.022.57331812
TCGGCGG32900.021.4894932
GAATGTC33850.021.405127
CGAGCAG13550.021.2031251
GTGGGAC34150.020.90932719
CGGGAAG63700.020.6261021
GTCGGCG34600.020.53480531
GGCGGCA35650.020.3224734
CGCAAAG20500.020.1675621
GGATCCG22250.020.13356437
CGCGAAA11050.019.975771
CGCCAAG12600.019.7429981
GAAGCGT36900.019.73183869
TGAAGCG37600.019.27139368
Produced by FastQC (version 0.11.5)