FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6846422
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99836414.582273777456312No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89580.1308420661186237No Hit
GGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81610.11920094905046752No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70680.10323640581898107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC62850.026.1884355
CGTATCA64700.025.86862456
CGCCGTA65700.025.80397253
CGAGATC11750.025.2223015
TCGCCGT69550.025.13027452
GCCGTAT69550.025.12934754
CTAGATC11150.025.0160525
GTATCAT64800.024.25829357
CAGATCG20350.023.6438585
GATCTCG75250.023.40974641
TCATTAA77400.023.10481360
AGATCTC77850.022.8989840
TCTCGGT76650.022.69871943
AAGATCG59900.022.3100626
CTCGGTG79400.022.22775544
TATCATT74250.021.92254658
GTCGCCG88800.021.81123751
TGGTCGC87200.020.96199249
TAGATCT87950.020.76915439
CATTAAA91600.020.55385861
Produced by FastQC (version 0.11.5)