FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9199330
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA54250.045.951941
ACACTCT59700.029.0891441
GCCACAC52350.026.886421
CGGGCTG4350.023.33281326
CCGGGCT4300.021.97625225
GCACCCA29950.020.8087861
AGGCCGC5400.020.74027616
GCACCAC41750.020.1269661
CACTCTT130650.016.454421
GGGCTGC6550.015.49583627
CGCCGGG7100.015.28134923
GGCCGCC7650.014.64019617
GTGGCGG8900.014.55071240
GAGGCCG7950.014.52797715
AGTGGCG9150.014.153151539
GCACACA19500.013.4663611
GGGATTC19700.013.32457432
TCGAGGC9500.013.26286113
CTGCTTG65900.013.22428764
CGCTTGC9800.013.2139923
Produced by FastQC (version 0.11.5)