FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8271863
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA43650.041.9507641
GCGTGCA20100.034.47694866
GCCGTCT360250.032.8959656
CTGCTTG321450.032.41343764
GAGATCG128400.032.332536
TGCCGTC389300.032.24828355
ATGCCGT375000.031.69538554
TATGCCG377800.031.67355353
CCGTCTT379500.031.47633457
GCTTGAA305000.031.35021266
TGCTTGA300600.030.99407865
GTATGCC390500.030.84063352
ATCTCGT383700.030.70345147
CGTATGC393150.030.59714351
AATCTCG386550.030.50424246
CTCGTAT391150.030.47621249
TCGTATG396550.030.33480650
CGGGCTG6350.030.31437526
TCTCGTA392150.030.27354248
CCGGGCT6300.029.99941825
Produced by FastQC (version 0.11.5)