FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4786468
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA21250.044.490621
GTCGGGG9000.040.4436462
GCCGTGT17250.037.5354956
GCGTGCA61600.037.1583566
GGGTGCA38200.036.8317366
TGCGTGC43750.035.59929365
GAGATCG141000.034.8561976
GTGCAAA107000.034.8357668
CGCGTGC16200.034.56721565
TGCCGGG20750.034.40895555
TCCGCGT19800.034.11548663
CGTGCAA68650.033.85220367
CTGCGTG47200.033.8128964
GGGTCGG9850.033.40035260
AAGATCG210950.033.3706446
CCGCGTG16550.033.2017664
GGTCGGG8650.033.17853561
CGAGATC50200.033.122415
GAAGATC61550.033.099925
GGGGTCG9850.033.0450359
Produced by FastQC (version 0.11.5)