FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5121046
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT11250.045.42130325
CGGGCTG11450.044.32224726
CGCCGGG11700.044.27260623
AGGCCGC11700.043.67390416
GGGCTGC12600.040.83250827
GAGGCCG13000.039.8449615
GCCCACA22750.039.7085651
GCCGGGC13200.039.2416324
TCGAGGC13400.038.91674413
GGCCGCC13650.037.43477617
CCGCCGG13800.037.28185322
CCTACGA12800.036.91367736
TCCTACG14400.035.97184435
ATCGAGG15750.033.33233312
CCGCCGC15450.033.29997619
CGCCGCC15400.033.1808220
CCCATGA16950.031.3992841
GGCTGCA16350.031.25319128
TATCGAG18950.027.51896511
CTACGAC16450.027.4467937
Produced by FastQC (version 0.11.5)