FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118778
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG4500.047.44355426
GCCCACA33500.046.0929761
CCGGGCT4750.044.94652625
AGGCCGC5300.041.60299316
CGCCGGG5500.039.45380423
GGGCTGC5600.038.12428327
CCTACGA5550.036.5758936
GGCCGCC6150.035.85298517
TCGAGGC6650.034.7361913
TCCTACG6100.033.27806535
GCCACAC30900.032.7476651
GAGGCCG6850.032.70011515
GCCGGGC6700.032.3874524
CCGCCGG6950.031.72602322
GCACCCA16600.031.0063191
GCGAGGG2650.029.05605716
CCCATGA8150.027.9252261
ATCGAGG8500.027.58771712
CTACGAC7450.026.30823137
GGCTGCA8200.025.60927428
Produced by FastQC (version 0.11.5)