FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6641944
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT89550.048.754811
GCCCACA76000.046.9436871
CGGGCTG8850.044.68873626
AGGCCGC9400.043.19032716
CGCCGGG9550.042.14609523
TCGAGGC11250.038.26563613
GCCACAC57850.037.1605031
GCCGGGC11150.036.0982324
GAGGCCG11550.036.0596415
CCGGGCT11200.035.62457725
CCTACGA10350.033.13970636
GCACCCA31600.032.574431
ATCGAGG14250.030.45532212
TCCTACG12650.028.49768635
CCGCCGG15000.027.06634922
GGCTGCA15300.026.30668328
GCGAGGG3900.025.12753116
CGAGGCC17100.024.97008914
CTACGAC12300.024.75599137
GCACCAC51600.024.6305161
Produced by FastQC (version 0.11.5)