FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5849443
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA79000.047.7299081
CCGGGCT5200.047.1146125
CGGGCTG5300.046.22565526
ACACTCT52050.045.066731
GCCACAC55450.044.008161
AGGCCGC5950.041.7636616
GGGCTGC6100.040.1632727
CGCCGGG6250.039.1990223
GGCCGCC6650.037.36748517
TCGAGGC7000.036.99907313
GCCGGGC6750.036.2953924
GAGGCCG7550.034.76733815
GCACCCA32350.032.5757331
CCGCCGG8550.029.063622
ATCGAGG9550.028.2191912
CCTACGA6400.027.34436
GGCTGCA9500.025.7890528
GCACCAC46050.025.6213631
TATCGAG11150.023.22811911
CACTCTT107950.023.1856082
Produced by FastQC (version 0.11.5)