FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8797348
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA60000.040.032211
CGGGCTG6200.038.95079826
CCGGGCT7350.033.8088225
GCCACAC48800.031.1391411
AGGCCGC8150.029.63128516
CGCCGGG8900.027.52751723
GGGCTGC9200.027.01030527
GCACCCA30300.026.0001961
GAGGCCG9850.024.87257815
GCCGGGC10550.024.21750524
GGCCGCC10650.023.00421517
TCGAGGC12200.020.36842713
CCGCCGG11900.020.29369422
GCACCAC43350.019.5462281
GGTCACG4850.018.76249512
CCTACGA12050.018.2985836
CCTATGC29150.017.2918784
TCCTACG13950.016.8097835
ATCGAGG16550.016.07217212
CTGCTTG51450.015.85000964
Produced by FastQC (version 0.11.5)