FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2875291
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA21500.044.944071
GGTGTTC9650.036.63129411
ACACTCT20900.036.3509031
GTGTTCC9950.034.11983512
CCTGAGA10700.030.7469717
TCCTGAG11300.030.66302516
CGGGCTG2100.028.33269126
GCGAGGG2750.027.99936516
GAGCGAG2700.027.22160514
GTTCCTG12600.026.94383414
GCGTGCA5400.023.98135666
CTGAGAT14350.023.65800318
AGGCCGC2600.022.88409816
CGCCGGG2600.022.88409823
GCCACAC23350.022.3409231
TGAGATC15300.022.1890419
TTCCTGA15950.021.94307915
GCACACA8000.021.8817351
GCACCCA12600.021.6733381
CACTCTT44950.020.9519441
Produced by FastQC (version 0.11.5)