FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404931
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA12630.3119049912207265TruSeq Adapter, Index 16 (97% over 40bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG10980.27115730828215173Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA9800.24201654108971649Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT9460.2336200488478284Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC9010.22250704441003527Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAG6800.1679298448377625Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC6330.15632292909162301TruSeq Adapter, Index 16 (100% over 39bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC5910.14595079161634944TruSeq Adapter, Index 16 (100% over 39bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC5680.1402708115703663Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCCA5490.13557865414107587Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTC5150.12718216189918774Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC5010.12372478274076323Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5010.12372478274076323Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT4920.1215021818532046TruSeq Adapter, Index 16 (100% over 37bp)
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCC4660.11508133484470194TruSeq Adapter, Index 16 (100% over 40bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC4450.10989526610706515TruSeq Adapter, Index 16 (100% over 38bp)
ACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC4450.10989526610706515TruSeq Adapter, Index 16 (100% over 38bp)
ACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4410.10890744349037243Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT4290.10594397564029429Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGA4080.10075790690265748Illumina Single End PCR Primer 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT13400.062.9696461
TACGTTG3550.061.12536214
CATACGT3650.060.40957612
CGTTGAG3550.060.1394716
CCATACG3900.056.53716311
ATACGTT4050.053.57901413
ACGTTGA4000.053.3737715
CCGGGCT608.367351E-1152.498825
AGGCCGC608.367351E-1152.498816
CGGGCGC200.00694943252.49879519
CGGGCTG651.8553692E-1048.46042626
TCGAGGC651.8553692E-1048.46042613
TCCTACG604.9276423E-946.665635
GCCCACA1450.045.8780141
GGGCTGC703.8380676E-1044.9989727
GGCCGCC703.8380676E-1044.9989717
CCGCCGG703.8380676E-1044.9989722
GCCGGGC757.548806E-1041.9990424
GTTGAGA5550.040.9900517
CCCATAC1650.040.30210510
Produced by FastQC (version 0.11.5)