FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8714845
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA30700.035.015631
GCGTGCA20750.032.72247768
GCGGCGT20600.028.54359
GGGTGCA25350.026.92273568
TGGCGGG23600.026.84257157
ATCGGGG70450.026.67784949
AATCGGG77600.026.47487448
TGCGTGC16850.026.37948867
AGGCCGC9050.026.29770716
CGGCGTC25900.026.21558260
TGCGGCG14650.025.80142458
CCGGGCT9500.025.05202525
GTGCAAA55950.025.0218870
GCGTCCG10850.024.83810862
GAGGCCG10150.024.82698415
ATCTCGG37800.024.72162649
CTCGGTT22500.024.42163351
GAATCGG86050.024.03790747
CGGGGGT25650.023.60566751
GTCGGGG9650.023.57469464
Produced by FastQC (version 0.11.5)