FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842072
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAAAA123400.045.9757570
CGTGAGA7300.044.5955733
TGAAAAA94750.044.4742170
GAAGATC50750.043.1787455
GCGTGAG3000.042.00732
GTGAGAT18600.041.4039154
AAAGATC59650.040.9028935
AAGATCG198200.040.4095546
GCCCACA7300.040.2853471
GTCCGCG4800.038.64520364
TGAGATC50650.038.4952775
GCGTGCA66750.038.4862668
CGAAGAT18100.037.906114
CTGCGTG53850.037.69669366
GTGCAAA88400.037.33536570
CTCGGAT7500.037.33272651
CGTCGGG3850.037.2721263
TGCGTGC44050.037.26386667
TGGCGTC20050.037.1814457
TCGGCGT17950.037.0467565
Produced by FastQC (version 0.11.5)