FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316334
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATC20990.15945800989718414TruSeq Adapter, Index 13 (97% over 39bp)
TGCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT20040.15224099658597284TruSeq Adapter, Index 13 (97% over 39bp)
CGCGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT19680.14950612838382965TruSeq Adapter, Index 13 (97% over 39bp)
CGCAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT19590.14882241133329382TruSeq Adapter, Index 13 (97% over 39bp)
TTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT17910.13605969305662544TruSeq Adapter, Index 13 (97% over 39bp)
TGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATC17460.13264110780394642TruSeq Adapter, Index 13 (97% over 39bp)
TCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATC17210.1307418937746803TruSeq Adapter, Index 13 (97% over 39bp)
CACGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT17080.1297543024794619TruSeq Adapter, Index 13 (97% over 39bp)
TTTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT16270.12360084902463965TruSeq Adapter, Index 13 (97% over 39bp)
TTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATC15830.12025823233313125TruSeq Adapter, Index 13 (97% over 39bp)
TGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATC15290.11615593002991642TruSeq Adapter, Index 13 (97% over 39bp)
ACGCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT14800.1124334705325548TruSeq Adapter, Index 13 (97% over 39bp)
CCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATC14610.11099006787031254TruSeq Adapter, Index 13 (97% over 39bp)
TTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT13820.10498855153783157TruSeq Adapter, Index 13 (97% over 39bp)
TTGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT13640.10362111743675997TruSeq Adapter, Index 13 (97% over 39bp)
CGTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT13360.1014939977239819TruSeq Adapter, Index 13 (97% over 39bp)
ACGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAT13260.10073431211227546TruSeq Adapter, Index 13 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA501.4551915E-1163.0390621
TGTCCGA2000.063.0390621
GTCGCGA401.0621079E-861.2879751
ATGCGCA401.0621079E-861.2879751
TGGGCGT401.0675649E-861.248465
AGCCCGA750.060.7042851
TCGGCGA1100.060.4920351
TTCGCGA2150.060.269911
CACGCTA700.060.0372051
AGCGCGA700.060.0372051
CACGCGA1300.059.2674981
CGAGCGA650.059.2674981
TGCGCTA601.8189894E-1258.3695071
CTCGGCG601.8189894E-1258.3318164
CGTGCGA553.6379788E-1157.3082431
CGGCGAG2350.056.6093252
GCGTGAG5700.056.5047842
CCGGGAA252.3838528E-456.034721
AGGACGA509.767973E-1056.034721
TGCGCGA1000.056.034721
Produced by FastQC (version 0.11.5)