FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8031305
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA49250.041.590961
ACACTCT50100.028.165451
CGGGCTG5700.025.7888426
GCCACAC55750.024.1804961
GCACCCA32400.023.3430771
CGCCGGG6450.022.79013623
GAGGCCG7100.021.68960615
AGGCCGC6900.021.30382316
CCGGGCT7400.019.86437625
GCACCAC37450.018.6993941
CCTACGA8400.014.582973536
TCGAGGC11350.014.49303413
GCACACA20300.014.4888071
GCGCGAT4351.8189894E-1214.48240213
CCGCCGG10450.014.40155922
GCCGGGC10450.014.06663924
CACTCTT127000.013.9782751
TGCGCGA4801.4551915E-1113.12467712
TCGCGCT20150.011.8139192
GGCCGCC13450.011.70974917
Produced by FastQC (version 0.11.5)