FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5358305
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA49400.048.2655071
ACACTCT52450.042.655211
AGGCCGC4600.041.84691616
GCCACAC39750.038.491161
GAGGCCG5450.035.9625215
GCACCCA21450.031.3393971
CGGGCTG5950.031.17579526
CCGGGCT6550.028.31999625
TCGAGGC7250.027.99939213
CGCCGGG7300.025.88984923
CACTCTT119400.023.8104931
GCACCAC34750.023.0726431
CCTACGA6750.022.81431836
TCCTACG7100.022.67556435
GCGCGAT2056.002665E-1122.19464113
TCCCGAA8200.021.7678215
AGTGGCG6500.021.53839539
ATCGAGG9850.021.31933412
GCCGGGC8800.020.68136824
GCGGAGC2101.9699655E-919.99956723
Produced by FastQC (version 0.11.5)