FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_vk7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_vk7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8699114
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA50650.045.9036561
GCCACAC40200.035.1024671
ACACTCT40350.026.727961
CGGGCTG4500.025.66627126
AGGCCGC5350.025.51348316
CCGGGCT4950.023.33297525
GCACCAC35750.021.1561361
GAGGCCG7150.019.58000815
GCACCCA31500.018.674861
GCGTGCA5900.017.79633766
GGGATTC19150.016.4487432
CGCCGGG7600.016.11817223
GCACACA18400.015.2240711
GGGGCGT5350.015.0464128
GTTGGGA21750.014.80428529
CACTCTT93100.014.2167631
GGGCGTC6200.014.1126059
CCTACGA6700.014.10426136
TCCTACG7750.013.99978535
TCGAGGC8350.013.83204413
Produced by FastQC (version 0.11.5)