FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6846422
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2321553.3908952734727715No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA39550.045.1537781
GCCACAC36000.036.8172841
AGGCCGC6900.034.3695116
CCGGGCT6800.033.9678125
GTCACAG96050.031.00554736
TCACAGT95200.030.75278937
ACACTCT38800.028.4969351
ACAGTCA70450.027.95370139
CCTACGA7050.027.89586436
CACAGTC73500.026.88485338
AGTTCCG33750.026.78080441
CGGGCTG8600.026.47869126
TCCTACG7700.026.44911435
GGGCTGC8950.025.83597227
CAGTCAA74850.025.74932540
ACTTAGG34400.025.45500639
CTTAGGC33950.024.9663140
GCACCCA22450.024.5477311
GTTCCGT35750.024.49376742
GAGATCG87550.024.0602786
Produced by FastQC (version 0.11.5)