FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8418939
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88288510.486891519228255No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113360.1346487960062426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG437850.028.7574870
AAAAAGG129600.022.88415770
GGGGAAA247150.016.63377660
GGGAAAA268300.015.56702161
GGAAAAA294950.015.01634862
GGGGGAA275750.014.98439359
AAAAGGG62550.013.54704470
GAAAAAA446200.011.6981163
GGGGGGA529300.09.23208758
AGAGGGG39050.07.35274270
AAAGGGG38500.07.275884670
GGAAGGG43150.07.14098970
AAAAAAA2824100.06.860072667
GGGAGCT5200.0079045056.759877762
GGGAGAA41400.06.534881659
AGGAGGG38600.06.440617670
GGAGGGG209150.06.31160870
TAGGGGG19750.06.1977251
AGCGGTT7953.4528965E-46.145248410
GAGGGGG192050.05.81611370
Produced by FastQC (version 0.11.5)