FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_AH9251-3H-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_AH9251-3H-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10240778
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131860112.875984617574954No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107640.10510920166417043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG1225950.035.65126470
AAAAAGG407250.024.19867770
GGGGAAA839400.017.4273660
GGGAAAA864350.017.19575361
GGGGGAA852000.017.15320259
GGAAAAA959150.015.93028762
AAAAGGG182800.014.56895870
GGGGGGA1103150.014.11089458
GAAAAAA1222250.013.42027363
GGGGTAA34400.08.54565160
AAAGGGG94400.07.562723670
GGGAGAA86000.07.528311359
GGGGGTA43400.07.499244758
AAAAAAA8353100.07.033565567
GGGGAGA98850.06.974512658
GGGGGAT34300.06.529954460
GTGGGAA46800.05.907524659
GGGGATA30400.05.756055461
GGAGAAA123950.05.731572660
GGTGGGA48400.05.567620858
Produced by FastQC (version 0.11.5)