FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_AH10677-3H-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_AH10677-3H-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12240153
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6958905.685304750684081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG765900.032.92160870
GGGGAAA477800.016.84622660
GGGAAAA484550.016.74216861
GGGGGAA488800.016.64610759
GGAAAAA575250.014.966269562
AAAAAGG354200.014.07944170
GGGGGGA733350.012.26907258
GAAAAAA784000.012.18211263
AAAAAAA4352450.07.573603667
GGGAGAA79900.06.307200459
AAAAGGG214950.06.28447570
GGGGAGA98450.06.00747458
GGGGGTA36350.05.776539359
AGGGGGG251850.05.09982967
GGGGGAT25105.0931703E-115.01937660
Produced by FastQC (version 0.11.5)