FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_AH10677-3H-Hop1mlChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_AH10677-3H-Hop1mlChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15930770
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3870852.429794667803251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG632400.024.63139770
GGGGAAA316950.014.15534960
GGGGGAA323900.013.90563759
GGGAAAA346500.013.170608561
GGGGGGA479200.010.6917258
GGAAAAA460200.010.53254462
AAAAAGG329650.08.3868270
GAAAAAA695600.08.1706563
AAAAAAA3555350.05.890307467
GGGGAGA87650.05.230493559
GGGAGAA86100.05.040131660
Produced by FastQC (version 0.11.5)