FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_AH10676-3H-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_AH10676-3H-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10371884
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3757513.6227844430192238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG571500.024.37202370
GGGGAAA275800.015.32819960
GGGGGAA278300.015.15277959
GGGAAAA298700.014.75078861
GGAAAAA380600.012.07315262
GGGGGGA421650.011.32089358
AAAAAGG282850.010.04672670
GAAAAAA587500.09.00080463
AAAAAAA3293900.05.80636767
GGGGAGA67500.05.28828560
GGGAGAA60800.05.23788859
Produced by FastQC (version 0.11.5)