FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_AH10676-3H-Hop1mlChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_AH10676-3H-Hop1mlChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15018172
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9857266.5635551384016635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG981100.039.1591170
GGGGAAA669300.017.15046360
GGGAAAA689600.016.8133561
AAAAAGG353800.016.48923970
GGGGGAA697500.016.2613959
GGAAAAA812950.014.94657362
GAAAAAA1071400.012.65090563
GGGGGGA1027850.012.42415858
AAAAAAA5936000.08.072367
GGGAGAA113550.07.211930860
AAAAGGG183200.07.01073370
GGGGAGA139550.06.7961459
GGAGAAA147450.05.957331760
GGGGGTA44900.05.76777459
AGGGGGG359900.05.63988867
GAGAAAA211600.05.14363962
AAGGGGG108350.05.13561667
GGGGTAA45900.05.03215659
Produced by FastQC (version 0.11.5)