FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_AH10675-3H-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_AH10675-3H-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14583163
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG510743135.02279306622301No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG334020.22904496095942972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG318520.21841626538769404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG290470.19918175501432717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG280300.1922079592746786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG258240.17708092544806636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG235680.1616110304739788No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG218670.14994689423686755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG215150.14753315175864112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG159410.10931099103808962No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGG158210.10848812428414878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG151860.10413378771121189No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG146870.10071203345940795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG3757350.049.48460870
AAAAAGG899850.042.88519770
AAAAGGG293750.033.33419470
GGGAAAA3256300.019.06291461
GGGGAAA3210400.019.01989660
GGAAAAA3434900.018.66572662
GGGGGAA3277450.018.5763359
GAAAAAA3898900.017.76744763
AAATAAG94500.017.22032270
ATAAAAG85750.015.79417670
AAAATAG125950.015.22658670
GGGGGGA4354350.015.0253258
AAAAATG161800.014.5781270
AATAAAG107950.013.74558870
AAAGGGG142000.012.3965270
GGGAGAA259800.011.6788959
GGAGAAA280600.011.58643160
AAAAGAG186900.011.32833970
AAAGAGG104600.011.17467570
AAATAGG44300.011.13872570
Produced by FastQC (version 0.11.5)