FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_AH10675-3H-Hop1mlChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_AH10675-3H-Hop1mlChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14437657
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10734457.435036031123332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG1025500.040.4287270
AAAAAGG357800.019.01398570
GGGGAAA716900.017.78370960
GGGAAAA753400.017.44269661
GGGGGAA740900.017.1651359
GGAAAAA894700.015.43106462
GAAAAAA1172200.013.22002163
GGGGGGA1103150.012.87357758
AAAAAAA5988900.08.841310567
GGGAGAA113450.08.39046859
AAAAGGG173200.08.36507270
GGGGGTA47350.07.98225459
GGGGAGA139200.07.34117259
GGAGAAA142750.06.91344960
GGGGTAA44100.06.8246760
AGGGGGG367600.06.216736367
GAGGGGG323350.05.844456766
GAGAAAA219150.05.669126561
AGAGGGG76750.05.654140566
AAATAAG87350.05.608960670
Produced by FastQC (version 0.11.5)