FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_AH10517-3H-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_AH10517-3H-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16495480
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6200203.7587266329927953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG942650.026.16198570
GGGGAAA478350.015.47343560
GGGGGAA484850.015.25877759
GGGAAAA510050.014.8417761
GGAAAAA646500.012.36959962
GGGGGGA738650.011.30930458
AAAAAGG455100.09.45065670
GAAAAAA969250.09.44943663
GGGAGAA104200.05.64239859
AAAAAAA5794050.05.631939467
GGGGAGA116450.05.559738658
Produced by FastQC (version 0.11.5)