FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n02_AH10517-3H-Hop1mlChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n02_AH10517-3H-Hop1mlChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10684564
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7896557.390615096694633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG718750.032.53917370
AAAAAGG279050.021.95922770
GGGGAAA507550.017.05838260
GGGGGAA519000.016.79667759
GGGAAAA528750.016.48748861
GGAAAAA603250.014.82841562
GGGGGGA674400.013.68388958
GAAAAAA763500.012.56867963
AAAAGGG135600.011.53614670
AAAAAAA3462200.09.20661667
AGGGGGG182600.07.4556151
GGGGGTA34250.07.35679959
GGGGTAA34500.06.4919959
AAAGGGG73450.05.76509570
GGGGGAT27900.05.644497460
AAAAGAG143400.05.320105670
GGGAGAA68650.05.301634359
GGGGAGA74550.05.257597458
Produced by FastQC (version 0.11.5)