FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8418939
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1227111.4575589631900172No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT105240.12500387519139883TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT99820.1185660093273036TruSeq Adapter, Index 16 (97% over 40bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90610.10762638855086133No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC90220.10716314728019766TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGT86440.10267327034914969TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG594850.067.71129611
AGAGCAC612650.067.430548
AGCACAC606100.067.31821410
GATCGGA584650.067.311281
CACACGT600150.067.2928112
ACACGTC592450.067.277913
CACGTCT588900.067.1887914
TCGGAAG622950.067.050363
ACGTCTG597700.066.8848115
ATCGGAA629500.066.8073652
CGGAAGA632150.066.433444
CGTCTGA601950.066.2885616
CTGAACT611150.065.4459119
TGAACTC616100.065.3417820
GAACTCC614100.064.7194221
CTCCAGT584800.064.56267524
GAGCACA639500.064.363299
AACTCCA623700.064.09920522
CCAGTCA534800.064.0414426
CAGTCAC495200.063.78449627
Produced by FastQC (version 0.11.5)