FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_AH9251-3H-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_AH9251-3H-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10240778
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC7407147.232985618866067TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC1048981.0243167071876766TruSeq Adapter, Index 2 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG982180.9590872880947131No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC359210.3507643657542425TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC346830.3386754404792292TruSeq Adapter, Index 2 (98% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302480.29536818394071235No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC273560.2671281420220222TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC232520.2270530617888602TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGG210390.20544337549354158TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGG128590.12556663175395463TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTTTGC112500.10985493484967646TruSeq Adapter, Index 2 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGC105220.10274609995451517TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGGTGG103960.10151572468419881TruSeq Adapter, Index 2 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCGT297100.068.1992650
TATCTCG1036800.068.02048539
TGCGTGA449750.067.8745158
TATGCCG927050.067.8081546
GTATGCC957950.067.791945
GCACACG1554250.067.77415511
ACACGTC1553600.067.67860413
GCCGGCG288900.067.62714449
CGTATGC938400.067.6265844
CACGTCT1552550.067.618114
CACACGT1557450.067.605712
ACGTCTG1552150.067.5791515
TGCCGGC316200.067.5775748
ATGCCGG331500.067.48884647
CTGCGTG381800.067.4872457
CTCGTAT884900.067.4633942
CGTCTGA1554600.067.45689416
GATCGGA1545600.067.443361
TCTCGTA894800.067.4132141
GCCGTCG279300.067.31995449
Produced by FastQC (version 0.11.5)