FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_AH9251-3H-Hop1mlChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_AH9251-3H-Hop1mlChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19397356
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1058860.5458785207633453TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG170100.053.6612115
ACACGTC173900.052.8912813
CACGTCT173600.052.86158414
GTATGCC165200.052.11720745
GCACACG178200.051.6935711
CACACGT182550.050.50010312
GATCGGA182050.049.781141
ATCTCGT162000.049.30111340
CGTCTGA186050.049.2113516
CAGTCAC185600.048.9540227
TCCAGTC185250.048.91413525
AGTCACT184550.048.8532528
CGTATGC175850.048.7618144
CTCGTAT164750.048.7543542
ATGCCGT170800.048.69019747
TGCCGTC163700.048.3217648
TCTCGTA165200.048.28256641
GAGCACA191900.048.0394489
CCAGTCA189750.047.90180226
GTCTGAA191700.047.8704817
Produced by FastQC (version 0.11.5)