FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_AH10677-3H-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_AH10677-3H-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12240153
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2636172.153706738796484TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC419510.34273264394652586TruSeq Adapter, Index 7 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG410590.335445153340812No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC211320.17264490076226988TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC174470.14253906793485344TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGG142740.11661618935645657TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATGC138180.1128907457284235TruSeq Adapter, Index 7 (97% over 45bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130520.10663265401992933No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC122980.10047260030164656TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG449200.065.6814139
GTATGCC368700.065.1184245
ACGTCTG814550.065.0907215
CACGTCT816550.065.0641714
ACACGTC818350.065.0579113
CACACGT820150.064.9364612
GTCTGAA822700.064.7139217
ATCTCGT400600.064.6070840
CAGTCAC820850.064.56315627
TCCAGTC818850.064.4895125
GCACACG826200.064.41859411
CCAGTCA819950.064.3907226
GAGCACA831950.064.330959
CGTATGC365100.064.3034244
TCTGAAC829050.064.1380418
GATCGGA823100.064.12451
CTCGTAT348700.064.0858142
AGTCACC820900.064.0433328
TCTCGTA353750.063.9921141
CAGATCA785600.063.89965434
Produced by FastQC (version 0.11.5)