FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_AH10677-3H-Hop1mlChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_AH10677-3H-Hop1mlChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15930770
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2251861.4135286618286498TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC740140.46459775641729806TruSeq Adapter, Index 12 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG235980.14812843321446484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC371900.063.48562245
ACGTCTG484050.063.33889415
CACGTCT486600.063.09327714
ACACGTC488600.062.89948713
CACACGT490650.062.61528812
TCCAGTC487350.062.32869725
GCACACG493850.062.26625411
ATCTCGT294100.062.08419440
GTCTGAA494300.061.9900717
CAGTCAC492250.061.8010827
CCAGTCA491800.061.8006926
AGTCACC488950.061.40931328
CCTTGTA473900.061.36508633
GAGCACA501900.061.3092089
CTCGTAT289550.061.2708642
TCTCGTA293450.060.95747441
CGTCTGA503850.060.87067816
TGAACTC505050.060.58783320
TCTGAAC506700.060.4866718
AGCACAC510400.060.33619310
Produced by FastQC (version 0.11.5)