FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_AH10676-3H-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_AH10676-3H-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10371884
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3133353.021003705787685TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG650730.6273980696274659No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280780.27071263041507215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC375550.062.59181247
TATGCCG369650.062.21790348
CGTATGC377250.062.12419546
ATCTCGT404000.061.95923642
CTCGTAT383200.061.8425344
ATGCCGT364050.061.6655449
TCTCGTA386300.061.55463843
TCGTATG382400.060.8919145
AATCTCG413850.060.84820641
TGCCGTC358400.060.48919750
ACGTCTG461850.060.20782515
CACAGTC448900.060.16764531
CACGTCT462500.060.13076814
CAATCTC417900.059.89000340
TCCAGTC461700.059.81860425
AGTCACA462950.059.81585328
ACAATCT421750.059.8080139
CCGTCTT330550.059.71955552
GTCACAG458700.059.67572429
GTCTGAA466900.059.66155217
Produced by FastQC (version 0.11.5)