FastQCFastQC Report
Fri 11 Sep 2020
HFVTNAFX2_n01_AH10676-3H-Hop1mlChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTNAFX2_n01_AH10676-3H-Hop1mlChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15018172
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT4226792.814450387170955TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT671840.4473513820456977TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGTGT635680.4232738844647671TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTGT551400.36715520370921306TruSeq Adapter, Index 14 (97% over 45bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG337450.22469445682204198No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTTT288860.19234031944766647TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGAT286930.19105520964868428TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGAT255060.16983425146549128TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGTAT155390.10346798531805336TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1213800.066.7573115
CACGTCT1216850.066.65613614
GATCGGA1209800.066.500051
CACACGT1222100.066.4847912
ACACGTC1221900.066.4784813
GAGCACA1223950.066.4643559
GCACACG1221300.066.46242511
GTCTGAA1221300.066.3645517
CCGTATC1142450.066.36211438
TCGGAAG1233000.066.049693
TATCTCG616850.066.0436241
TCCAGTC1216400.066.0371625
AGCACAC1232900.066.0074210
AGTCACA1218200.065.9225728
CCAGTCA1220850.065.8339526
CAGTCAC1223650.065.8177327
AGAGCAC1238000.065.812068
TCTGAAC1231400.065.76337418
TGAACTC1229900.065.7019620
CGTCTGA1234750.065.6246316
Produced by FastQC (version 0.11.5)